Journal: Cell Death & Disease
Article Title: NOS2-derived low levels of NO drive psoriasis pathogenesis
doi: 10.1038/s41419-024-06842-z
Figure Lengend Snippet: a Expression of the IL17-regulated and psoriasis-associated genes CXCL1, CXCL8, IL-6, MMP1, MMP12 and S100A8 in human keratinocytes treated with the NOS2 inhibitor aminoguanidine (AG) and IL-17 in combination with TNFα. n = 3 b CXCL1 expression was analyzed in keratinocytes in response to different concentrations of the NO donor DETA-NONOate (0.1-1000 µM). n = 3. Statistical analysis was performed using Student’s t -test ( a ) or one-way ANOVA with Dunnett’s multiple comparisons test ( b ). **** p < 0.0001, *** p < 0.001, ** p < 0.01, * p < 0.05.
Article Snippet: Analyses of MMP1 and CXCL1 protein levels in conditioned medium were performed using the human pro-MMP1 and CXCL1/GROα Quantikine ELISA immunoassay kits (DMP100, DGR00B, R&D Systems, Minneapolis, MN), according to the manufacturer’s instructions.
Techniques: Expressing